Wear particles and surface topographies are modulators of osteoclastogenesis in vitro.
نویسندگان
چکیده
Prosthetic and osteosynthetic implants from metal alloys will be indispensable in orthopedic surgery, as long as tissue engineering and biodegradable bone substitutes do not lead to products that will be applied in clinical routine for the repair of bone, cartilage, and joint defects. Therefore, the elucidation of the interactions between the periprosthetic tissues and the implant remains of clinical relevance and several factors are known to affect the longevity of implants. Within this study, the effects of metal particles and surface topography on the recruitment of osteoclasts was investigated in vitro in a coculture of osteoblasts and bone marrow cells. The cells were grown in the presence of particles of different sizes and chemical composition or on metal discs with polished or sandblasted surfaces, respectively. At the end of the culture, newly formed osteoclasts were counted. Osteoclastogenesis was reduced when particles were added directly to the coculture. The effect depended on the size of the particles, small particles exerting stronger effects than larger ones. The chemical composition of the particles, however, did not affect the development of osteoclasts. In cocultures grown on sandblasted surfaces, osteoclasts developed at higher rates than they did in cultures on polished surfaces. The data demonstrate that wear particles and implant surfaces affect osteoclastogenesis and thus may be involved in the induction of local bone resorption and the formation of osteolytic lesions, leading eventually to the loosening of orthopedic implants.
منابع مشابه
Wear particles enhance autophagy through up-regulation of CD147 to promote osteoclastogenesis
Objective(s): The study aimed to uncover the underlying mechanism linking wear particles to osteoclast differentiation, and we explored the effect of titanium particles of different sizes on CD147 expression and autophagy in macrophages. Materials and Methods: Effects of titanium particles on CD147 and RANKL mRNA were detected by QPCR; protein level of CD147 and Beclin-1 were detected by Wester...
متن کاملThe influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts.
Orthopedic implant failures are often associated with peri-implant osteolysis. Particles generated from the wear process have been suspected to play an important role in this situation. Indeed, the peri-implant osteolysis could be due to the presence of particles stimulating the osteoclastogenesis process. We hypothesize then that the presence of a low particle concentration positively influenc...
متن کاملProduction of Dispersed Ceramic Nano-Particles in Al Alloy Using Friction Stir Processing
In this research, surface composite layers containing nano sized TiB2, Al2O3-TiB2, ZrO2 and CNT particles have been fabricated on Aluminum alloy substrates by friction stir processing. The effects of different processing variables such as number of passes and strengthening particle distribution, hardness, and wear properties of surface nano...
متن کاملProduction of Dispersed Ceramic Nano-Particles in Al Alloy Using Friction Stir Processing
In this research, surface composite layers containing nano sized TiB2, Al2O3-TiB2, ZrO2 and CNT particles have been fabricated on Aluminum alloy substrates by friction stir processing. The effects of different processing variables such as number of passes and strengthening particle distribution, hardness, and wear properties of surface nano...
متن کاملStudy on tool wear and surface roughness in end milling of particulate aluminum metal matrix composite: Application of response surface methodology
Metal matrix composites have been widely used in industries, especially aerospace industries, due to their excellent engineering properties. However, it is difficult to machine them because of the hardness and abrasive nature of reinforcement elements like silicon carbide particles (SiCp).In the present study, an attempt has been made to investigate the influence of spindle speed (N), feed rate...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of biomedical materials research. Part A
دوره 72 1 شماره
صفحات -
تاریخ انتشار 2005